000			02446nam a2200193Ia 4500
005			20151006141706.0
008			150525s2013xx 000 0 und d
041			_aeng
082			_a1663,T
100			_aZeenat Salah-ud-din _98399
110			_cDr. Muhammad Yasir Zahoor _95785
245			_aDna Quantification Of Blood Stains Recovered From Washed Fabrics
260			_c2013
502			_aBloodstains are extremely important forensic evidences for DNA profiling. Bloodstains recovered at crime scene can help to identify and confirm the credentials of the victim. Criminal can make all efforts to destroy and hide possible evidence at crime scene therefore these bloodstains on the crime place can hardly provide any evidence against the culprit. The criminal will try to take away or change clothes with the bloodstains of the victim. Once perpetrator has removed the bloodstains, culprit will feel secure and satisfied. But as a suspect if clothes are recovered with washed bloodstains and forensic evidence of DNA of the victim can be recovered from the washed clothes and can provide strong evidence against suspect. Present study has been planned to quantiate DNA from 10 different types of bloodstained fabrics with possible ways of washing to hide the blood from fabrics. 100 ìl of preservative blood was used in creating blood spot on fabric. After that dried bloodstained fabrics were hand washed with tap water and in detergent for 5 and 10mins and air dried at room temperature (25°C- 30°C) in laboratory. DNA was extracted through Chelex® 100 extraction procedure from these fabrics and analyzed on Real time PCR kit i.e. Quantifiler™ Human DNA Quantification kit which is specific for Human DNA. Research study showed there was no statistically significant difference in DNA concentrations among different washing methods (p-value = 0.318). There was statistically significant difference in DNA concentrations among different types of fabrics divided into two categories (p-value = 0.001, 0.002). Research findings showed that cellulose fibers recovered highest quantity of DNA, followed by artificial fibers and then protein fibers (cellulose >artificial>protein fiber) despite their washing methods. Thus provides a lead in solving such type of criminal cases.
650			_aDepartment of Forensic Science _95695
700			_aDr. Muhammad _94698
700			_aDr. Muhammad Wasim _95181
942			_cTH
999			_c3371 _d3371