000			02959nam a2200181Ia 4500
005			20151006142835.0
008			150525s2013 xx 000 0 und d
041			_aeng
082			_a1698,T
100			_aZanira Shakoor _98457
110			_cDr. Jawad Nazir _98397
245			_aPreparation & Evaluation Of Combined Pre & Fmd Vaccines For Small Ruminants
260			_c2013
502			_aPPR is an acute, febrile and contagious viral disease of small ruminants caused by Morbillivirus. Similarly, FMD is another highly contagious viral disease of cloven hoofed animals that affects more than 33 species of domestic and wild animals. Although disease severity is relatively higher in large ruminants but small ruminants might also play an important role in the epidemiology and transmission of FMD. Mass vaccination is the most effective mean to control viral diseases like PPR and FMD in small ruminants. The present study was designed to evaluate the immune response of goats to various monovalent and bivalent as well as adjuvant and non adjuvant PPRV and FMD "O" virus vaccines. A total of nine groups of animals each comprising 5, were inoculated with various formulations of the vaccines. Serum samples were collected at the beginning and at 1, 2, and 3 months post vaccination. Mean neutralizing antibody titers (MNA) against PPRV and MNA along with complement fixing (CF) antibody titers against FMD "O" in the serum samples were measured to test the efficacy of the vaccines. Live attenuated wet, gel and oil based PPR vaccines induced 229.2 (±112.79), 253.6(±83.05), and 424 (±182.06) MNA titers in the goats after 3 months of vaccination. Similarly, animals inoculated non-adjuvant, gel based, and oil adjuvant FMD "O" vaccines developed 20(±4.35), 186.2 (±65.39), and 285.8 (±63.80) MNA and 0.4 (±0.894),22.4(±8.76) and 25.6(±8.7) CF antibody titers at 3 months post vaccination (PV). Bivalent (PPRV and FMDV) non adjuvant vaccine induced 249.6(±3.130) and 27.2(±14.53), gel based vaccine induced 306 (±99.82) and 296.2 (±58.21) while the oil based vaccine provoked 417.8 (±141.56) and 286 (±97.13) MNA titres against PPRV and FMD "O" virus respectively, at 3 months PV. Results of present study demonstrate that live attenuated PPRV vaccines can induce better immune response in comparison to killed FMD "O" virus vaccine. Addition of adjuvants is helpful to enhance the immunogenicity of the added antigens. While adjuvant bivalent vaccines containing PPRV and FMD "O" virus can effectively provoke equivalent antibody response against both of the immunogens in the vaccine. Antibody titee in response to Bivalent vaccine of PPR and FMD seotype"O" containing oil as an adjuvant was superior to gel adjuvant and non adjuvant bivalent vaccines. There was poor immune response of goats to non adjuvant FMD"O" vaccines.
650			_aDepartment of Microbiology _94609
700			_aProf. Dr. Khushi Mohammad _98458
942			_cTH
999			_c3406 _d3406