000			01903nam a22002057a 4500
005			20161024151251.0
008			161024b2016 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2587-T
100			_aJaveria Zafar (2014-VA-222) _926629
110			_cDr. Asif Nadeem _95126
245			_aSequence Analysis Of Mitochondrial Atpase 8/6 Gene Variants In Sindh Ibex (Capra Aegagrus Blythi)
260			_c2016.
300			_a74p.;
502			_aATPase 8/6 gene plays a vital role in survival of an organism by generating energy in the form of ATP synthase. Considering the importance of ATPase8/6 gene in energy generating, present study has been designed to characterize this gene in Sindh ibex. The characterization of ATPase8/6 gene might be helpful for deriving phylogenetic relationship among different species and identifying new functions among the related species. Tissue/blood samples (n=15) were collected from Kirthar National Park, Sindh. Standard DNA extraction method was used for DNA extraction. PCR primers were designed by Primer3 software and amplification of gene was done by Polymerase Chain Reaction. PCR product was sequenced bi-directionally by Big Dye TM Terminator on ABI 3130XL Genetic Analyzer. Multiple sequence alignment was performed for polymorphism identification. Genetic diversity was calculated by using DNAsp. Phylogenetic analysis using the MEGA6 software package and an equally weighted maximum parsimony analysis was performed using the close-neighbor-interchange algorithm. The results indicated that Sindh ibex ATPase8/6 gene was highly similar to Capra caucasica. The results of this data might be helpful in designing effective conservation strategies of different species of wild animal.
650			_aMolecular Biology and Biotechnology _926630
700			_aDr. Maryam Javed _911391
700			_aProf. Dr. Abu Saeed Hashmi _96670
942			_cTH
999			_c9481 _d9480