Study On The Diversity And Antibiotic Resistance Profile Of Uropathogenic Bacteria In Human And Dogs (Record no. 10542)

000 -LEADER
fixed length control field 03732nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170213114124.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 170213b2016 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2646-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Umbreen Aslam Khan (2009-VA-222)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Arfan Ahmad
245 ## - TITLE STATEMENT
Title Study On The Diversity And Antibiotic Resistance Profile Of Uropathogenic Bacteria In Human And Dogs
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2016.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 63p.;
502 ## - DISSERTATION NOTE
Dissertation note Annually more than 150 million people globally face the problem of Urinary tract infection (UTI) and treatment cost goes up to 6 billion dollars. UTI is more common in women as compared to men. The infection is caused by the invasion of pathogens in the urinary tract and produces the inflammatory response of urothelium. In Pakistan, urinary tract infections are increasing with antibiotics resistant among the uropathogens day by day. Duration of treatment is also getting increased which subsequently increased the medication cost. This situation is possibly due to emergence of antibiotic resistant uropathogenic bacteria. Like humans, in dogs same situation is prevailing. The study therefore was designed for isolation identification and antibiotic resistance assessment of UTI causing bacteria.
Bacterial pathogens causing UTI in dogs and human are becoming more resistant to antibiotic use .To evaluate the diversity and antibiotic resistance of uropathogenic bacteria, a total of 80 urine samples were collected in sterile containers. A total of 15 urine samples were taken from each gender of human and dogs with UTIs and 5 from each gender of healthy human and dogs. Samples were cultured for isolation and confirmed by biochemical tests and their antibiotic resistance pattern was checked by Kirby baur disk diffusion test.
Samples that were taken from UTI males, E.coli, Proteus Spp., klebsiella Spp. and Staph aureus were isolated from 93%, 6.67%,20% and 53% of processed samples whereas from UTI females E.coli, Proteus Spp.,Klebsiella spp. and Staph aureus was isolated 100%,13%,33%and 40% of samples respectively.
Similarly 60 % Staph aureus was isolated from healthy males and females urine samples.
Summary
58
While Samples that were taken from UTI dogs E.coli, Proteus Spp.,klebsiella Spp. and Staph aureus was isolated from 66.7%, 6.67%,33% and 26.6% of processed samples whereas Samples that were taken from bitches, E.coli, Proteus Spp., klebsiella Spp. and Staph aureus was isolated from 73%,13%,26.6% and 26.6% of samples respectively.
In case of healthy dogs and bitches urine smalpes 60% and 80% of Staph aureus was isolated
Antibiotic resistance pattern of isolates E.coli, Proteus Spp., klebsiella Spp. and Staph aureus from human (male and female) samples showed resistance to ceftriaxone, Levofloxecine, and mild type of resistance to Ofloxacin while sensitive to ciprofloxacin and sulfamethazole.
The antibiotic resistance pattern of isolates E.coli, Proteus Spp., klebsiella Spp. and Staph aureus from dogs and bitches samples showed high resistance to Lincomycin and kanamycin, and lower resistance to norfloxacin and doxycyclin while sensitive to ciprofloxacin, amoxiclin.
Conclusion
It is concluded that UT infection mainly caused by Gram negative (Enterobacteracea) .Among the (Enterobacteracea) member only E.coli was most abundantly isolated from human and dogs UTI samples most effective antimicrobial agents were urixin, sulfamethazole and ciprofleoxacin against isolates of human origin. For dogs ciprofloxacin amoxiclin are best against gram negative and positive bacterial isolate.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Microbiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Fareeha Akhtar
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Muhammad Nasir
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2017-02-13 2646-T 2646-T Thesis


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