Production Of Alginate By Azotobacter Vinelandll By Submerged Fermentation
By: Muhammad Awais
| Miss Shagufta Saeed.
Contributor(s): Miss Asma Waris | Miss Sehrish.
Material type: 
BookPublisher: 2011Subject(s): Institute of Biochemistry & BiotechnologyDDC classification: 1348,T Dissertation note: Alginate is a linear coploymer of 1, 4,-beta-D-mannuronic acid and alpha -L-guluronic acid. They are usually obtained from brown algae which contains of D-mannuronic and L-guluronic acids. Alginates are important polysaccharides with many application in the food, pharmaceutical, textiles and paper industry.
Azotobacter vinelandii is a gram negative, aerobic and free-living soil microbe which play an important role in the nitrogen cycle in nature. A. vinelandii was grown on wheat bran for the production of alginate. The optimal condition like substrate:water ratio, pH, incubation period, ionic concentration (Calcium, magnesium, sodium and phosphate ions) and addition of corn steep liquor was carried out for the production of alginate by A. vinelandii through submerged fermentation. The cell biomass and alginate concentration was determined to estimate the concentration of alginate. The alginate thus produced was estimated by determining its concentration spectrophotometrically at 546 nm. The effect of pH, incubation period, ionic concentration and addition of nitrogen source like corn steep liquor was analysed by statistical linear regression method.
Alginate is an important polymer with wide applications in pharmaceutical, food and textile industry. It has also medical application in encapsulating hormones.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1348,T (Browse shelf) | Available | 1348,T |
Alginate is a linear coploymer of 1, 4,-beta-D-mannuronic acid and alpha -L-guluronic acid. They are usually obtained from brown algae which contains of D-mannuronic and L-guluronic acids. Alginates are important polysaccharides with many application in the food, pharmaceutical, textiles and paper industry.
Azotobacter vinelandii is a gram negative, aerobic and free-living soil microbe which play an important role in the nitrogen cycle in nature. A. vinelandii was grown on wheat bran for the production of alginate. The optimal condition like substrate:water ratio, pH, incubation period, ionic concentration (Calcium, magnesium, sodium and phosphate ions) and addition of corn steep liquor was carried out for the production of alginate by A. vinelandii through submerged fermentation. The cell biomass and alginate concentration was determined to estimate the concentration of alginate. The alginate thus produced was estimated by determining its concentration spectrophotometrically at 546 nm. The effect of pH, incubation period, ionic concentration and addition of nitrogen source like corn steep liquor was analysed by statistical linear regression method.
Alginate is an important polymer with wide applications in pharmaceutical, food and textile industry. It has also medical application in encapsulating hormones.
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