Level Of Amylase From Human Saliva Deposited On Fruit First Bite Mark
By: Umar Draz
| Ms. Sehrish Firyal.
Contributor(s): Dr. Mohammad Ashraf Tahir | Prof. Dr. Tahir.
Material type: 
BookPublisher: 2013Subject(s): Department of Forensic ScienceDDC classification: 1755,T Dissertation note: Saliva is colorless fluid which consists of epithelial cells, enzymes, non enzyme protein and inorganic components. Saliva is secreted by three glands in mouth. One is parotid gland, second is submandibular gland and third is sublingual gland. There are two types of amylases in human. One is salivary amylase, while other is pancreatic amylase. The salivary amylase is secreted by salivary gland while pancreatic amylase is secreted by pancreas. The salivary amylase is present in saliva, perspiration and breast milk. Pancreatic amylase is present in blood, feces and urine. Saliva stain is very important at crime scene for forensic investigation. Majority of techniques used for detection of saliva are based upon the presence of salivary amylase. Human saliva can serve for identification. One can extract DNA from saliva stain and generate DNA profile, whereby individual can be identified who is a source DNA profile that is generated from saliva stain. In present study level of salivary amylase was determined from human saliva deposited on fruit with first bite mark. Apple, peach and apricot were selected for this experiment. Ten males and ten females were selected to bite on fruits. The time interval was used as variable for determining the level of amylase. The time intervals were 0 hour, 12 hours, 24 hours, 36 hours and 48 hours. Samples were collected from bite mark area on fruit. The samples collected from apples and apricot pits were positive for amylase activity till 48 hours. The samples collected from peach were positive till 12 hours. The samples collected from peach were negative after 24 hours. This research indicates that salivary DNA could be found on bite mark area on apple and apricot pit till 48 hours.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
|
UVAS Library Thesis Section | Veterinary Science | 1755,T (Browse shelf) | Available | 1755,T |
Browsing UVAS Library Shelves , Shelving location: Thesis Section , Collection code: Veterinary Science Close shelf browser
Saliva is colorless fluid which consists of epithelial cells, enzymes, non enzyme protein and inorganic components. Saliva is secreted by three glands in mouth. One is parotid gland, second is submandibular gland and third is sublingual gland. There are two types of amylases in human. One is salivary amylase, while other is pancreatic amylase. The salivary amylase is secreted by salivary gland while pancreatic amylase is secreted by pancreas. The salivary amylase is present in saliva, perspiration and breast milk. Pancreatic amylase is present in blood, feces and urine. Saliva stain is very important at crime scene for forensic investigation. Majority of techniques used for detection of saliva are based upon the presence of salivary amylase. Human saliva can serve for identification. One can extract DNA from saliva stain and generate DNA profile, whereby individual can be identified who is a source DNA profile that is generated from saliva stain. In present study level of salivary amylase was determined from human saliva deposited on fruit with first bite mark. Apple, peach and apricot were selected for this experiment. Ten males and ten females were selected to bite on fruits. The time interval was used as variable for determining the level of amylase. The time intervals were 0 hour, 12 hours, 24 hours, 36 hours and 48 hours. Samples were collected from bite mark area on fruit. The samples collected from apples and apricot pits were positive for amylase activity till 48 hours. The samples collected from peach were positive till 12 hours. The samples collected from peach were negative after 24 hours. This research indicates that salivary DNA could be found on bite mark area on apple and apricot pit till 48 hours.
There are no comments for this item.