1.
Prevalence And Biochemical Studies On Cattle Suffering From Babesiosis In District Swabi,Khyber Pukhtoonkha
by Naveed Khan | Muhammad Yasin Tipu | Dr.Habib-ur-Rehman | Dr.Muhammad Younas Rana.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The present study was designed to diagnose Babesiosis in cattle in District Swabi Khyber Pukhtoonkhwa, to check its prevalence, determine its effect on the liver, kidney function tests and to check the role of supportive therapy. For this purpose blood sample was collected from 100 animals suspected to be suffering from Babesiosis in 20 different villages in district Swabi. Selection of animals was based on clinical signs like haemoglobinuria, temperature and jaundice of Babesiosis. The infection was confirmed by blood smears using Giemsa staining technique. The blood smears showed Babesia piroplasms, which were like double pear shaped or like signet-ring shaped. On the basis of microscopic examination the overall prevalence of Babesiosis in cattle in District Swabi was recorded as 10%. The blood of animals showing Babesia was analyzed in chemistry analyzer using commercially available kits.The values of SGPT, SGOT, Albumin, Total Bilirubin and Creatinine were increased while the values of Total protein an Albumin were decreased as compared to the normal reference values.
Bebesia positive animals Group A were divided into two Group B and Group C. Group B was only treated with antibabesial drug Imizol and Group C was treated with Imizol along with supportive drug i.e. liver tonic and diuretic (Hepasel+Lasix). After 5 days of treatment the plasma of treated animals was again analyzed for biochemical parameters and was compared before and after treatment (with and without supportive drug). The biochemical analysis showed that the biochemical indicators were normalizing in Group B and coming near to normal in Group C. The study is helpful in better understanding of the pathogenesis, supporting therapy and the effect of disease on the affected animals. This study showed that Babesiosis damaged the kidney and liver and the use of supportive drug along with specific drug was effective to recover the animals from infection.
Availability: Items available for loan: UVAS Library [Call number: 1267,T] (1).
2.
Optimization Of Multiplex Pcr For The Simultaneous Detection Of Babesia Caballi Theileria Equi And Trypanosoma
by Muhammad Zeeshan Khalid | Prof. Dr. Asim aslam | Dr. Yasin tipu | Prof. Dr.Habib ur rehman.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2006,T] (1).
3.
Effect Of Different Dietary Concentration Of Moringa Oleifera On Different Biological Parameters In Broilers
by Shumaila Ashraf | Dr. Muhammad Shahbaz Yousaf | Prof. Dr.Habib-ur-Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2159,T] (1).
4.
Influence Of Chicory Root Powder And Zinc Sulfate On Growth Performance, Relative Visceral Weights, And Histomorphology Of Muscles And Bones In Broilers.
by Amber zafar(2015-VA-1054) | Dr.Saima ashraf | Dr. Saima ashraf | Dr.Hafsa zaneb | Dr.Habib ur rehman.
Material type: Publisher: 2017Dissertation note: Several feed additives are under investigation for improving the health and performance of broilers in this post-antibiotic era. Phytogenic feed additives like, chicory root powder (CRP)and minerals e.g. ZnSO4 has been extensively studied for this purpose in poultry. Literature indicated their positive influence on performance, mineralization of bone and muscle morphology in broiler. However, little is known regarding their utility in combination particularly with respect to their effect on performance. Therefore, present study was designed to report the individual and combined effects of CRP and ZnSO4 on performance, relative weights of viscera, histomorphometry of bones and muscles in broilers.
150 d-old broilerwerekept in environmentally controlled experimental sheds in, University of Veterinary and Animal Sciences, UVAS, Lahore. Ethical committee approved the whole procedure and proper techniques and protocols were used to handle the birds. The birds were randomly divided into six groups with five replicates (n=5) in each group. Broilerswere raised according to standard management protocol for 35 days. Group-CONT (control) received corn-based basal diet (BD), Group-2C (BD+2%CRP), Group-4C (BD+4%CRP), Group-Zn60 (ZnSO4 60mg/kg of diet), Group-2C+Zn60 (2%CRP+ZnSO4 60g/kg), Group-4C+Zn60 (4%CRP+ZnSO4 60m/kg).
During the trial, feed intake and mortality of the birds was measured on daily basis whereas body weights were taken on weekly basis. Weekly feed conversion efficiency was calculatedby dividing the feed intake by body weight. Footpads of the birds were observed for any dermatitis or inflammation. On day 35, two birds from each replicate were randomly selected and slaughtered by exsanguination. Skin and viscera of birds were removedmanually. The carcass and the relative weight of visceral organswere measured. The left and right tibia of each bird was detached as drumstick. Right tibial bone was boiled for ten minutes and then cooled down at room temperature. Afterwards flesh on the drumsticks was removed andbone were allowed to air dry at room temperature. Samples of breast (Pectoralis major) and thigh (Biceps femoris) were collected for determination of pH, WHC and histomorphometery. For histological slide preparation breast muscle sample were processed by paraffin embedding technique and stained by Hematoxylin& Eosin. Histomorphometrywas done by using software (ProgRes®2.1.1 Capture Prog Camera Control Software).One-way analysis of variance was used to analyze the data. Group differences were compared by the Duncan’s Multiple Range Test. Difference between groups was considered significant at P < 0.05.
The results revealed that at the end of the experimentgroup 4C+Zn60 had lesser (p<0.05) FI as compared to the other groups. All supplemented groups had increased (p<0.05) BW compared to that of CONT group. Moreover, a lower (p<0.05) FCR was observed in the 4C and 4C+Zn60 groups during 5th week. All the groups scored zero when observed for the footpad lesions and there was no difference in carcass weights of broilers in all the groups. All supplemented groups showed increased (p<0.05) weight of gizzard and thymus than that of the CONT group except group Zn60. Group 2C showed increased (p<0.05) weight of proventriculus compared to all of the groups. The relative weight of small intestine was increased in group 2C and 2C+Zn60. All supplemented groups showed increased (p<0.05) right tibial bone weight and length than that of the CONT group.Diaphyseal diameter, medullary canal diameter and thickness of bony wall were increased (p<0.05)in all supplemented groups except the Zn60 group. W/L index were higher (p<0.05) in all supplemented groups of CRP and ZnSO4 as compared to the CONT group. Cartilage thickness were also increased (p<0.05) in all supplemented groups as compared to the CONT group.The results revealed thatthe group 2Cand 4C showed increased (p<0.05) pH of breast and thigh muscles and increased(p<0.05) water-holding capacity and better meat quality as compared to the other groups. Histological analysis of pectoralis majormuscles revealed increased (p<0.05)muscle fascicle and fiber diameter and cross sectional area in all supplemented groups when compared to the CONT group.Results of muscle fascicles and fibers of the bicepsfemorismuscles showed increased(p<0.05) diameter as compared to the CONT group. There was no significant difference in cross section area of muscle fascicles and fibers of the bicepsfemorisof broilers.
Conclusion
Dietary supplementation of 2% and 4% CRP individually and in combination with 60mg ZnSO4 improved the performance, relative weight of gizzard, proventriculus, thymus and small intestine,meat quality and bone strength of the broilers.
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5.
Effect Of Bisphenol-A On Glucose Uptake And Electrophysiology Of Isolated Ileum Of Rabbit
by Hina Rafiq (2014-VA-921) | Dr.Imtiaz Rabbani | Dr.Habib ur Rehman.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: A major nutritional problem these days is the biological and chemical contamination of food with environmental chemicals. Bisphenol-A (BPA) is one of a chemical which is produce worldwide and cause food contamination. BPA has many genomic and non-genomic effects and target various organs of both human and animals and cause hazardous effects. BPA cause other diseases such as angina, coronary heart disease, heart attack, stroke, asthma, emphysema, chronic bronchitis, arthritis, thyroid hormone disruption, liver damage and disrupted beta cell function. It has adverse effects on the homeostasis of glucose which leads to the metabolic disorders like insulin resistant type 2 diabetes. BPA also affect the barrier function of small intestine. The current study was aimed to investigate the effect of BPA on the barrier function of intestine.
Bisphenol-A (BPA) wasdisrupt glucose uptake and perturb the gut barrier function. Our study aimed to find out the effect of Bisphenol-A on the electrophysiology of ileum of small intestine of rabbit.For this purpose twenty healthy rabbits of uniform age and weight were selected and slaughtered at slaughter house of UVAS. Ileum was taken from the intestinal part and rinsed with standard buffer. Serosallayer was removed and tissue was mounted in Ussing Chamber. After incubation period of 15-20mins different groups were treated with different concentrations of BPA anddivided into four groups (A, B, C, and D), each having five rabbits. Group A, was control. In group B, tissue was treated with Bisphenol-A 5 µM. Group C was treated with 15 µM of Bisphenol-A, and group D was treated with 30µM of Bisphenol-A. After treatment the electrophysiological parameters like short circuit current (Isc), conductance (Gt) and potential difference (mV)was observed. BPA affected the electrophysiology of isolated ileum of rabbit by decreasing the short circuit current. The decrease of current between the groups is significant (p value < 0.05). Iscwas significantly decrease between the groups containing 5µМ BPA and control group.Gtwas significantly different from group 5µМ and group 15µМ of BPA.mV was significantly different from control group and group 15µМ of BPA.
Statistical analysis was conducted with statistical packages for social sciences (SPSS). The data were analyzed using one way analysis of variance (ANOVA). Post hoc test was carried out using Tukey. P value was considered significant at P<0.05. The result of this study showed that BPA 5µM effects the electrophysiology of intestine as compared to other groups (15 µM and 30 µM). Suggesting a possible role of BPA in affected intestinal barrier function and glucose uptake.
Availability: No items available
